Table 1 In vitro specific activities of enzymes in genetically modified C. glutamicum strains and original strain C. glutamicum JL-6 as well as wild-type C. glutamicum ATCC13032

ATCC13032

2.17 ± 0.11

nd

0.04 ± 0.00

0.43 ± 0.02

1.96 ± 0.32

JL-6

1.61 ± 0.06

nd

0.07 ± 0.03

0.66 ± 0.07

1.10 ± 0.17

JL-68

1.92 ± 0.02

nd

0.06 ± 0.02

0.70 ± 0.04

1.16 ± 0.14

JL-68∆gltA

0.05 ± 0.00

0.06 ± 0.01

0.05 ± 0.00

0.27 ± 0.03

0.65 ± 0.11

JL-68∆ramA

0.22 ± 0.03

0.04 ± 0.00

0.05 ± 0.01

0.78 ± 0.07

1.24 ± 0.17

JL-68∆P1gltA (or JL-69)

0.31 ± 0.02

0.05 ± 0.00

0.04 ± 0.02

0.83 ± 0.05

1.31 ± 0.21

JL-68∆P12gltA

0.05 ± 0.00

0.07 ± 0.01

0.05 ± 0.00

0.25 ± 0.08

0.57 ± 0.06

JL-68P_dapA-L1 gltA

0.13 ± 0.01

0.09 ± 0.04

0.06 ± 0.02

0.73 ± 0.04

1.14 ± 0.07

JL-68∆prpC1

1.89 ± 0.06

< 0.0

< 0.0

0.69 ± 0.05

1.16 ± 0.13

JL-68 ∆prpC2

1.91 ± 0.09

< 0.01

< 0.01

0.69 ± 0.09

1.19 ± 0.16

JL-68∆prpC1∆prpC2

1.88 ± 0.03

–

–

0.68 ± 0.06

1.15 ± 0.11

JL-68∆gltA∆prpC1

< 0.01

–

–

nd

nd

JL-68∆gltA∆prpC2

≤ 0.01

–

–

nd

nd

JL-68∆gltA∆prpC1∆prpC2

ng

nd

nd

nd

nd

JL-68∆gltA∆prpC1prpR^G977A

0.21 ± 0.02

0.32 ± 0.13

0.33 ± 0.15

0.79 ± 0.01

1.27 ± 0.17

JL-69P_dapA-L1 gdh

0.21 ± 0.03

0.04 ± 0.01

0.31 ± 0.08

0.23 ± 0.07

0.21 ± 0.04

JL-69P_tac gdh

0.24 ± 0.02

0.05 ± 0.00

0.31 ± 0.11

0.92 ± 0.10

1.74 ± 0.17

JL-69P_tac-M gdh

0.27 ± 0.05

0.05 ± 0.01

0.32 ± 0.09

1.00 ± 0.07

2.56 ± 0.24

JL-69P_tuf gdh

0.31 ± 0.02

0.07 ± 0.03

0.34 ± 0.14

1.11 ± 0.21

3.98 ± 0.32

JL-69P_sod gdh

0.30 ± 0.01

0.06 ± 0.00

0.33 ± 0.06

1.04 ± 0.18

3.07 ± 0.15