Fig. 6

Cylindrotheca closterium protein visualization by two-dimensional gel electrophoresis. Total proteins were extracted from the marine diatom Cylindrotheca closterium from 18 h (a), and 24 h (b) dried biomass samples using SDS-DTT buffer. Sample proteins (120 μg) were loaded per gel, and standard protein markers were run on each gel for molecular mass determination. A total of 9 proteins were excised and subjected to MALDI-TOF–MS analyses, out of which only six were identified with high confidence and attributed to C. closterium