Microbial Cell Factories

Table 3 Composition of complete modified Poolman medium and the simplified minimal medium for B. pumilus DSM 18097

From: Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium

Ingredients	Complete mod. Poolman medium
Ingredients	Concentration (g/L)
Main components
Glucose	10^a
Sodium acetate	1^a
(NH₄)₂SO₄	7.49^a
MOPS acid	20.93^a
K₂HPO₄	1.7^a
Trace elements
MgCl·6H₂O	0.427^a
CaCl₂·2H₂O	0.007^a
MnCl₂	0.016^a
FeCl₃·6H₂O	0.005^a
FeCl₂	0.005^a
ZnSO₄·7H₂O	0.009^a
CoSO₄·7H₂O	0.005^a
CuSO₄·5H₂O	0.004^a
(NH₄)₆Mo₇O₂₄·4H₂O	0.003^a
Amino acids
Alanine	0.24
Arginine	0.125
Aspartic acid	0.42
Cysteine	0.13^a
Glutamate	0.5
Glycine	0.175
Histidine	0.15^a
Isoleucine	0.21
Leucine	0.475
Lysine	0.44
Methionine	0.125
Phenylalanine	0.275
Proline	0.675
Serine	0.34
Threonine	0.225
Tryptophan	0.05
Tyrosine	0.25
Valine	0.325
Threonine	0.225
Tryptophan	0.05
Tyrosine	0.25
Valine	0.325
Nucleobases/nucleosides
Adenine	0.01
Guanine	0.01
Inosine	0.005
Xanthine	0.01
Thymidine	0.005
Uracil	0.01
Vitamins
Ascorbic acid	0.5
Biotin	0.003^a
Nicotinic acid	0.001
Calciumpantothenate	0.001
p-Aminobenzoic acid	0.01
Pyridoxamine HCl	0.005
Pyridoxine HCl	0.002
Riboflavin	0.001
Pyridoxine HCl	0.002
Thiamine HCl	0.001
Vitamin B12	0.001
Orotic acid	0.005
Folic acid	0.001

^aComponents of the simplified minimal medium, containing only essential nutrients

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ISSN: 1475-2859

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