Fig. 8

Cultivation of B. pumilus DSM 18097 in modified Poolman medium containing all nucleobases/nucleosides and amino acids specified in Table 3 supplemented with biotin and vitamins from group 7. Oxygen transfer rate during cultivation of B. pumilus DSM 18097 in the complete modified Poolman medium (10 g/L glucose) as a reference and without any vitamins, a with all vitamins from group 7 and group 8 as well as with biotin and b biotin with each vitamin from group 7 (folic acid and ascorbic acid) separately as specified in Table 2. For clarity only every second measuring point over time is represented by a symbol. For all cultivations biological duplicates are shown. Culture conditions: 48-well Round Well Plate, filling volume 700 µL, shaking frequency 1100 rpm, shaking diameter 3 mm and temperature 37 °C