Fig. 6

Cultivation of B. pumilus DSM 18097 in modified Poolman medium containing all nucleobases/nucleosides and amino acids specified in Table 3 supplemented with different groups of vitamins. Oxygen transfer rate during cultivation of B. pumilus DSM 18097 in the complete modified Poolman medium (10 g/L glucose) and without any vitamins and in the medium containing only vitamins from a group 6, b group 7 and c group 8. These groups are specified in Table 2. For clarity only every second measuring point over time is represented by a symbol. For all cultivations biological duplicates are shown. Culture conditions: 48-well Round Well Plate, filling volume 700 µL, shaking frequency 1100 rpm, shaking diameter 3 mm and temperature 37 °C