Skip to main content
Fig. 5 | Microbial Cell Factories

Fig. 5

From: Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium

Fig. 5

Cultivation of B. pumilus DSM 18097 in modified Poolman medium containing all nucleobases/nucleosides and vitamins specified in Table 3 supplemented with every single amino acid from the glutamate and serine family. Oxygen transfer rate during cultivation of B. pumilus DSM 18097 in the complete modified Poolman medium (10 g/L glucose) as a reference and without any amino acids and with each amino acid separately from a group 1 (glutamate family) and b group 3 (serine family). These groups are specified in Table 1. For clarity only every second measuring point over time is represented by a symbol. For most cultivations biological duplicates are shown. Culture conditions: 48-well Round Well Plate, filling volume 700 µL, shaking frequency 1100 rpm, shaking diameter 3 mm and temperature 37 °C

Back to article page