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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium

Fig. 3

Cultivation of B. pumilus DSM 18097 in modified Poolman medium lacking different groups of nutrients. Oxygen transfer rate during cultivation of B. pumilus DSM 18097 in the complete modified Poolman medium (10 g/L glucose) defined in Table 3 as a reference and without nucleobases/nucleosides, without vitamins or without amino acids. For clarity only every second measuring point over time is represented by a symbol. For most cultivations biological duplicates are shown. Culture conditions: 250 mL shake flask, filling volume 10 mL, shaking frequency 350 rpm, shaking diameter 50 mm and temperature 37 °C

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