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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium

Fig. 2

Cultivation of B. pumilus DSM 18097 in two types of minimal medium with and without addition of different complex compounds. Oxygen transfer rate during cultivation of B. pumilus DSM 18097 in a, b V3 mineral medium (20 g/L glucose) with different concentrations of a yeast extract and b peptone. For clarity only every second measuring point over time is represented by a symbol. For the cultivations biological duplicates are shown. c Oxygen transfer rate during cultivation in modified Poolman medium (10 g/L glucose). Culture conditions: 250 mL shake flask, filling volume 10 mL, shaking frequency 350 rpm, shaking diameter 50 mm and temperature 37 °C

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