Fig. 10

Cultivation of B. pumilus DSM 18097 in simplified minimal medium lacking ascorbic acid and one of the amino acids cysteine or histidine. Oxygen transfer rate during cultivation of B. pumilus DSM 18097 in a complete modified Poolman medium (10 g/L glucose) defined in Table 3 as a reference and with cysteine, histidine, biotin and with as well as without ascorbic acid. b B. pumilus DSM 18097 was also cultivated in the simplified minimal medium with biotin and either histidine or cysteine to reduce the ingredients in the medium to a minimum. For clarity only every second measuring point over time is represented by a symbol. For all cultivations biological duplicates are shown. Culture conditions: 48-well Round Well Plate, filling volume 700 µL, shaking frequency 1100 rpm, shaking diameter 3 mm and temperature 37 °C