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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium

Fig. 1

Overview of cultivation steps and cultivation systems. First preculture was performed in TB medium and inoculated with a single colony from a LB agar plate. For experiments with a two-step preculture, a second preculture in V3 mineral medium was inoculated with cells from the first preculture. Both precultures were carried out in 250 mL flasks in a RAMOS device. The main culture was performed in three cultivation systems: RAMOS device (250 mL shake flasks), separate 250 mL shake flasks for offline sampling and µRAMOS device (48-well Round Well Plates). The RAMOS and µRAMOS device allow an online measurement of the oxygen transfer rate (OTR) of the culture

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Microbial Cell Factories

ISSN: 1475-2859

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