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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Characterizing the effect of expression of an acetyl-CoA synthetase insensitive to acetylation on co-utilization of glucose and acetate in batch and continuous cultures of E. coli W

Fig. 4

Acetate metabolism in E. coli and results of the gene expression analysis. Green arrows indicate overexpression of acs with Leu-641 residue changed to proline under control of a constitutive promoter. Solid black lines show glucose catabolism and acetate formation pathways, dashed lines indicate reverse reactions. Grey boxes show fold change of expression levels: A, ACS_L641P vs. VC at dilution rate 0.2 h−1, B, ACS_L641P vs. VC at dilution rate 0.65 h−1, C, 0.65 h−1 vs. 0.2 h−1 for VC, D, 0.65 h−1 vs. 0.2 h−1 for ACS_L641P. acs, acetyl-CoA synthetase, phdc, pyruvate dehydrogenase complex, poxB, pyruvate oxidase, pta, phosphotransacetylase, ackA, acetate kinase, yjcH, putate membrane protein, actP, acetate permease, cra, catabolite repressor activator, crp, cAMP receptor protein. The fold changes given represent the mean of two biological and three technical replicates. For visualization reasons, standard errors and p-values are not shown, but can be seen in Additional file 2: Table S1

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Microbial Cell Factories

ISSN: 1475-2859

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