Table 4 Saccharomyces cerevisiae strains used in this study
From: Engineering de novo anthocyanin production in Saccharomyces cerevisiae
Name | Relevant genotype | Contains plasmid | Origin |
|---|---|---|---|
IMK393 | MATalpha ura3-52 his3-Δ1 leu2-3112 trp1-289MAL2-8cSUC2 Δaro3::loxP ARO4G226S pdc6Δ::loxP pdc5Δ::loxP aro10Δ::loxP |  | [7] |
IMX106 | IMK393 pyk2Δ::LEU2-TDH3P-atCHI1-CYC1T TPI1p-atCHS3-ADH1T, TEF1P-at4CL3-TEF1T | pUDE172 | [7] |
IMX106-F | IMX106 | pF | This study |
IMX106-FD | IMX106 | pFD | This study |
IMX106-FDA | IMX106 | pFDA | This study |
IMX106-FDA3 | IMX106 | pFDA3 | This study |
PATW002 | IMK393 | pCPW002 | This study |
PATW003 | IMK393 | p414-TEF1p-Cas9-CYC1t | This study |
PATW004 | PATW003 X-2::TEF1P-At4CL3-TEF1T TPI1P-AtCHS3-ADH1T TDH3P-AtCHI1-CYC1T | p414-TEF1p-Cas9-CYC1t | This study |
PATW005 | PATW004 XII-2::TDH3P-AtPAL1-CYC1T TPI1P-coC4H-ADH1T PGI1P-coCPR1-PGI1T TEF1P-coCHS3-TEF1T | p414-TEF1p-Cas9-CYC1t | This study |
PATW006 | PATW005 can1Δ::GAP1P-coF3H-TEF1T TPI1P-coANS-PGK1T TEF1P-coGhDFR-CYC1T PGI1P-co3GT-ADH1T | p414-TEF1p-Cas9-CYC1t | This study |
PATW011 | IMK393 | pCPW006 | This study |
PATW012 | IMK393 | pCPW007 | This study |
PATW034 | PATW006 exg1Δ::TEF1P-coCHS3-TEF1T | p414-TEF1p-Cas9-CYC1t | This study |
PATW044 | PATW034 spr1Δ::TDH3P-coTAL1-CYC1T | p414-TEF1p-Cas9-CYC1t | This study |
PATW066 | PATW005 | Â | This study |
PATW072 | PATW044 tsc13Δ::TSC13P-coMdERC-TSC13T | p414-TEF1p-Cas9-CYC1t | This study |
PATW079 | PATW006 | Â | This study |
PATW080 | PATW044 | Â | This study |
PATW076 | PATW072 | Â | This study |