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Table 3 Product formation in S. cerevisiae strains PATW066, PATW079, PATW080 and PATW076 in shake flask cultures

From: Engineering de novo anthocyanin production in Saccharomyces cerevisiae

 

IMX106a

PATW066

PATW079

PATW080

PATW076

PATW079

PATW080

PATW076

[Concentration]Ext µM

[Concentration]Int µmol/gCDW

Coumaric acid

25

2.4 ± 0

2.8 ± 0

21.2 ± 9

51.6 ± 5

0.02 ± 0.00

0.24 ± 0.01

0.50 ± 0.02

Phloretic acid

200

167.2 ± 7

200.2 ± 2

139.5 ± 8

–

–

–

–

Naringenin

46.5

40.0 ± 4

1.8 ± 0

1.9 ± 0

3.6 ± 0

0.11 ± 0.07

0.15 ± 0.01

0.32 ± 0.02

DHK

–

–

28.8 ± 2

33.2 ± 3

92.5 ± 4

0.45 ± 0.04

0.71 ± 0.06

1.71 ± 0.12

Kaempferol

–

–

–

0.3 ± 0

0.6 ± 0

0.11 ± 0.01

0.17 ± 0.03

0.36 ± 0.03

K3G

–

–

8.3 ± 1

7.5 ± 1

16.1 ± 1

0.09 ± 0.03

0.14 ± 0.02

0.22 ± 0.01

Pelargonidin

–

–

–

–

–

0.003 ± 0.001

0.005 ± 0.001

0.011 ± 0.002

P3G

–

–

–

–

–

–

–

–

  1. The strains were grown in shake-flasks with 50 mL SMG and extracellular metabolite concentration of coumaric and phloretic acids, naringenin, dihydrokaempferol (DHK), kaempferol, kaempferol 3-O-glucoside (K3G), pelargonidin and pelargonidin 3-O-glucoside (P3G) expressed in µM were measured by HPLC in supernatant sampled after 140 h of cultures at 30 °C. Growth and production time courses of extracellular metabolites can be found in Additional file 3. Intracellular concentrations expressed µmol/gCDW were extracted and measured by HPLC. Data represent the average ± mean deviation of independent biological triplicates
  2. – not detected
  3. a Data from [7] (140 h of growth of IMX106)