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Table 2 Product formation in S. cerevisiae strains PATW002, PATW011 and PATW012 in shake flask cultures

From: Engineering de novo anthocyanin production in Saccharomyces cerevisiae

 

PATW002

PATW011

PATW012

PATW002

PATW011

PATW012

[Concentration]Ext µM

[Concentration]Int µmol/gCDW

DHK

60.7 ± 2.3

77.0 ± 3.1

43.3 ± 0.7

2.25 ± 0.17

1.64 ± 0.18

0.84 ± 0.04

Kaempferol

2.7 ± 0.2

1.5 ± 0.2

5.0 ± 0.2

1.76 ± 0.07

0.55 ± 0.04

1.45 ± 0.05

K3G

50.3 ± 3.6

12.4 ± 0.3

33.3 ± 1.9

1.58 ± 0.14

0.26 ± 0.02

0.49 ± 0.05

Pelargonidin

–

–

–

0.066 ± 0.01

0.019 ± 0.00

0.026 ± 0.00

P3G

–

–

–

–

–

–

  1. These strains express coGhDFR, coMtDFR1 and coAaDFR, respectively in combination with coF3H, coANS, co3GT genes. The strains were grown in shake-flasks with 50 mL SMNar (1.5 mM naringenin) and extracellular metabolite concentration of dihydrokaempferol (DHK), kaempferol, kaempferol 3-O-glucoside (K3G), pelargonidin and pelargonidin 3-O-glucoside (P3G) expressed in µM were measured by HPLC in supernatant sampled after 140 h of cultures at 30 °C. Growth and production time courses of extracellular metabolites can be found in Additional file 2. Intracellular concentrations expressed in µmol/gCDW were extracted and measured by HPLC. Data represent the average ± mean deviation of independent biological triplicates
  2. – not detected