Table 1 Strains and plasmids used in this study
Name | Relevant characteristics | References |
|---|---|---|
E. coli strains | ||
 JM109 | Wild type | [24] |
 JM109SG | JM109 Δsad ΔgabD | [24] |
 JM109 (pBHR68) | P3HB producing strain | This study |
 JM109 (pBHR68 + pBBR1MCS-2) | P3HB producing strain | This study |
 JM109 (pBHR68 + pMCS-pta-ackA) | P3HB producing strain | This study |
 JM109 (pBHR68 + pMCS-acs) | P3HB producing strain | This study |
 JM109SG (p68orfZ + pMCSH5) | P3HB4HB producing strain | This study |
 JM109SG (p68orfZ + pMCSH5-pta-ackA) | P3HB4HB producing strain | This study |
 JM109 (p68-pct-CAB + pBBR1MCS-2) | PHBV producing strain | This study |
 JM109 (p68-pct-CAB + pMCS-pta-ackA) | PHBV producing strain | This study |
 JM109 (p68-pct-CAB + pMCS-pta-ackA-prpP) | PHBV producing strain | This study |
Plasmids | ||
 pBHR68 | phaCAB expression plasmid | [25] |
 p68orfZ | orfZ inserted into pBHR68 | [24] |
 pEn | Expression vector, Ppdc promoter | [24] |
 pBBR1MCS-2 | Broad-host-range vector | [26] |
 pMCSH5 | Ppdc-sucD-4hbD inserted into pBBR1MCS-2 | [24] |
 p68-pct-CAB | pct and phaCAB expression plasmid | [27] |
 pEn-pta-ackA | pta-ackA inserted into pEn | This study |
 pEn-acs | acs inserted into pEn | This study |
 pEn-prpP | prpP inserted into pEn | This study |
 pMCS-pta-ackA | Ppdc-pta-ackA inserted into pBBR1MCS-2 | This study |
 pMCS-acs | Ppdc-acs inserted into pBBR1MCS-2 | This study |
 pMCSH5-pta-ackA | Ppdc-pta-ackA inserted into pMCSH5 | This study |
 pMCS-pta-ackA-prpP | Ppdc-pta-ackA-Ppdc-prpP inserted into pBBR1MCS-2 | This study |