Fig. 1From: Metabolic engineering of Escherichia coli for the synthesis of polyhydroxyalkanoates using acetate as a main carbon sourceP3HB, P3HB4HB, and PHBV synthesis pathway in engineered Escherichia coli. Genes: acs, acetyl-CoA synthetase; pta, phosphotransacetylase; ackA, acetate kinase; phaA, β-ketothiolase; phaB, acetoacetyl-CoA reductase; phaC, PHA synthase; sucD, succinate semialdehyde dehydrogenase; 4hbD, 4-hydroxybutyrate dehydrogenase; orfZ, 4-hydroxybutyryl-CoA:CoA transferase; sad and gabD, succinate semialdehyde dehydrogenase of E. coli; pct, propionyl-CoA transferase; prpP, propionate permease. The reactions and intermediates shown in green are associated with 3HB precursor biosynthesis, those in gold with 4HB precursor biosynthesis, those in purple with 3HV precursor biosynthesis, and those in blue with acetate assimilation and TCA cycleBack to article page