Table 2 The time course of the transformation of 16α,17α-epoxy-pregnenolone and 16α-methoxy-pregnenolone by P. lanosocoeruleum
Substrate | Rt (min.) | Steroidal compounds identified in the mixture (%)a | Time of transformation (h) | |||
|---|---|---|---|---|---|---|
12 | 24 | 48 | 72 | |||
16α,17α-Epoxy-pregnenolone (9) | 6.38 | 16α,17α-Epoxy-pregnenolone (9) | 100 | 93 | 19 | 7 |
8.30 | 3β,16α-Dihydroxy-17a-oxa-d-homo-androst-5-en-17-one (10) | – | 2 | 22 | 26 | |
8.59 | 16α-Hydroxy-17a-oxa-d-homo-androst-4-en-3,17-dione (11) | – | 1.5 | 31 | 48 | |
9.06 | 3β,16α-Dihydroxy-17a-oxa-d-homo-5α-androstan-17-one (12) | – | 1 | 7 | 8 | |
9.86 | 16α-Hydroxy-17a-oxa-d-homo-5α-androstan-3,17-dione (13) | – | 0.5 | 5 | 8 | |
7.63 | 16α,17α-Epoxy-progesterone (14)b | – | – | – | – | |
6.96 | 16α-Hydroxyandrostenedione (15)b | – | – | – | – | |
16α-Methoxy-pregnenolone (16) | 6.23 | 16α-Methoxy-pregnenolone (16) | 33 | 8 | 1 | – |
7.65 | 16α-Methoxy-progesterone (17) | 66 | 86 | 87 | 79 | |
8.13 | (20R)-20-Hydroxy-16α-methoxy-pregn-4-en-3-one (18) | 1 | 4 | 12 | 19 | |