Table 1 Primers and plasmids used or constructed in this study
Name | Description | Reference |
|---|---|---|
Primer | Sequence (restriction sites are underlined) | |
F_BF_fkp (BamHI) | ATAGGATCCATGCAAAAACTACTATCTTTGCCTCCTAATC | This study |
R_BF_fkp (SalI) | ATAGTCGACTTATGATCGTGATACTTGGAATCCCTTATCCG | This study |
F_BT_fkp (BamHI) | ATAGGATCCATGCCGGAGCCGATCTGCTGTTTCCTTC | This study |
R_BT_fkp (SalI) | ATAGTCGACTTAGCTTCTCGATACCTGTAATCC | This study |
F_BO_fkp (BamHI) | ATAGGATCCATGCAAAAGTTATTATCCTTACCAC | This study |
R_BO_fkp (SalI) | ATAGTCGACTTAGCTTCTTGAAACCTGAAGTCCCTTGTCAG | This study |
F_LAC12 (SpeI) | TCTAGAGCGGCCGCACTAGTGCCACCATGGCAGATCATTCGAGCAG | This study |
R_LAC12 (SalI) | TCTAGAGCGGCCGCGTCGACTTAAACAGATTCTGCCTCTG | This study |
F_fucT2 (SpeI) | GG ACTAGTATGGCCTTTAAGGTCGT | This study |
R_fucT2 (XhoI) | CCGCTCGAGTTAGGCATTATACTTTTGAGACTTAACT | This study |
Plasmid | ||
pRS423GPD | HIS3, GPD promoter, CYC1 terminator, 2 µ origin, and Ampr | [25] |
pRS425GPD | LEU2, GPD promoter, CYC1 terminator, 2 µ origin, and Ampr | [25] |
pRS426GPD | URA3, GPD promoter, CYC1 terminator, 2 µ origin, and Ampr | [25] |
pRS425GPD_BF_fkp | pRS425GPD harboring fkp from B. fragilis 9343 | This study |
pRS425GPD_BT_fkp | pRS425GPD harboring fkp from B. thetaiotaomircon | This study |
pRS425GPD_BO_fkp | pRS425GPD harboring fkp from B. ovatus | This study |
pRS423GPD_LAC12 | pRS425GPD harboring LAC12 from K. lactics | This study |
pRS426GPD_fucT2 | pRS425GPD harboring fucT2 from H. pylori | This study |