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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Production of a human milk oligosaccharide 2′-fucosyllactose by metabolically engineered Saccharomyces cerevisiae

Fig. 6

Identification of 2′-fucosyllactose (2-FL) produced by fed-batch fermentation of the engineered yeast, Saccharomyces cerevisiae D452-2_LFF. The mass spectra of a 2-FL and b difucosyllactose produced during fed-batch fermentation were obtained by LC/MS. Strain D452-2_LFF indicates S. cerevisiae D452-2 harboring fkp coding for l-fucokinase/guanosine 5′-diphosphate-l-fucose phosphorylase, fucT2 coding for α-1,2-fucosyltransferase, and LAC12 coding for lactose permease

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