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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Production of a human milk oligosaccharide 2′-fucosyllactose by metabolically engineered Saccharomyces cerevisiae

Fig. 5

Production of 2′-fucosyllactose (2-FL) by fed-batch fermentation of the engineered Saccharomyces cerevisiae D452-2_LFF strain. Ethanol was fed intermittently when depleted. During the fermentation, the cell density (OD600) and the concentrations of glucose, ethanol (EtOH), and 2-FL were monitored using HPLC. All points are the means of duplicate experiments. Strain D452-2_LFF indicates S. cerevisiae D452-2 harboring fkp coding for l-fucokinase/guanosine 5′-diphosphate-l-fucose phosphorylase, fucT2 coding for α-1,2-fucosyltransferase, and LAC12 coding for lactose permease

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