Fig. 5From: Production of a human milk oligosaccharide 2′-fucosyllactose by metabolically engineered Saccharomyces cerevisiaeProduction of 2′-fucosyllactose (2-FL) by fed-batch fermentation of the engineered Saccharomyces cerevisiae D452-2_LFF strain. Ethanol was fed intermittently when depleted. During the fermentation, the cell density (OD600) and the concentrations of glucose, ethanol (EtOH), and 2-FL were monitored using HPLC. All points are the means of duplicate experiments. Strain D452-2_LFF indicates S. cerevisiae D452-2 harboring fkp coding for l-fucokinase/guanosine 5′-diphosphate-l-fucose phosphorylase, fucT2 coding for α-1,2-fucosyltransferase, and LAC12 coding for lactose permeaseBack to article page