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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Production of a human milk oligosaccharide 2′-fucosyllactose by metabolically engineered Saccharomyces cerevisiae

Fig. 4

Identification of 2′-fucosyllactose (2-FL) produced by batch fermentation of the engineered yeast Saccharomyces cerevisiae D452-2_LFF strain by GC/MS. a The overlaid GC/MS chromatogram at 363 m/z, showing the unique daughter ions of 2-FL. Mass spectra of b 2-FL standard and c 2-FL produced by strain D452-2_LFF. S. cerevisiae D452-2_LFF, S. cerevisiae D452-2 harboring fkp coding for l-fucokinase/guanosine 5′-diphosphate-l-fucose phosphorylase, fucT2 coding for α-1,2-fucosyltransferase, and LAC12 coding for lactose permease; Control, the D452-2_LFF_Control strain harboring three empty plasmids

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