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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Production of a human milk oligosaccharide 2′-fucosyllactose by metabolically engineered Saccharomyces cerevisiae

Fig. 3

Batch fermentation profiles of engineered Saccharomyces cerevisiae D452-2_LFF in a modified Verduyn medium with 20 g/L glucose, 2 g/L fucose, and 2 g/L lactose in 50 mM potassium hydrogen phthalate buffer (pH 5.5) at 30 °C and 250 rpm for 48 h. During fermentation, the cell density (OD600) and concentrations of glucose, ethanol (EtOH), and 2′-fucosyllactose (2-FL) were monitored by HPLC. All data points are the means of experimental data from duplicate fermentations. Strain D452-2_LFF indicates S. cerevisiae D452-2 harboring fkp coding for l-fucokinase/guanosine 5′-diphosphate-l-fucose phosphorylase, fucT2 coding for α-1,2-fucosyltransferase, and LAC12 coding for lactose permease

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