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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Production of a human milk oligosaccharide 2′-fucosyllactose by metabolically engineered Saccharomyces cerevisiae

Fig. 2

Production of 5′-diphosphate-l-fucose (GDP-l-fucose) in engineered Saccharomyces cerevisiae. Engineered strains cultured in yeast synthetic complete (YSC) medium composed of 6.7 g/L yeast nitrogen base with 20 g/L glucose, 0.69 g/L CSM-Leu, 5 g/L l-fucose, and 2 mM MgCl2 in 50 mM potassium hydrogen phthalate buffer (pH 5.5) at 30 °C and 250 rpm for 36 h. a The overlaid HPLC chromatograms of D452-2_BF_FKP and control strains. b Comparison of intracellular GDP-l-fucose concentrations depending on the origin of the gene, fkp, coding for l-fucokinase/GDP-l-fucose phosphorylase (FKP). Control (D452-2_FKP_Control), S. cerevisiae D452-2 harboring pRS425GPD; BF (D452-2_BF_FKP), S. cerevisiae D452-2 harboring pRS425GPD_BF_fkp; BT (D452-2_BT_FKP), S. cerevisiae D452-2 harboring pRS425GPD_BT_fkp; BO (D452-2_BO_FKP), S. cerevisiae D452-2 harboring pRS425GPD_BO_fkp; ND, not detectable; DCW, dry cell weight. All data points are the means of experimental data from duplicate fermentations

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