Fig. 6

Pentose phosphate pathway modulation and its effect on ʟ-Ornithine production. a Relative tkt and zwf mRNA levels in strains SO15 (SO12 carrying AAV taq inserted into the C-terminal of Pgi and RBS together with start codon T1A replacement in front of zwf) and SO16 (SO15 carrying P _tac promoter inserted in front of tkt operon). Samples at the 12 h time point were subjected to transcript analysis during fermentation cultivation. b ʟ-Ornithine production curves for strains SO12 (black square), SO14 (SO12 carrying AAV taq inserted into the C-terminal of Pgi) (red cycle), SO15 (blue upper triangle), and SO16 (pink lower triangle). c Growth profiles of these strains. d Glucose consumption curves. Samples were collected per 12 h for fermentation parameter determination. Data represent the average values and standard deviations from three individual experiments