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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Optimization of ʟ-ornithine production in recombinant Corynebacterium glutamicum S9114 by cg3035 overexpression and manipulating the central metabolic pathway

Fig. 3

Blocking acetate biosynthesis by inserting a terminator into the upstream region of pta and cat, and its effect on ʟ-ornithine production. a Relative pta and ack mRNA levels in SO1 and SO5 (SO1 carrying modulation in pta). Samples at the 12 h time point were subjected to transcript analysis during fermentation cultivation. b Relative cat mRNA levels in SO1 and SO6 (SO1 carrying modulation in cat). c ʟ-Ornithine production by the engineered strains SO1, SO5, and SO6. d Cell growth and glucose consumption. Samples were collected per 12 h for determining the fermentation parameters. Results of standard deviations present in three individual experiments

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