Production of the compatible solute α-d-glucosylglycerol by metabolically engineered Corynebacterium glutamicum

Table 5 Production of α-GG with the trehalose synthesis deficient strain C. glutamicum ΔotsA ΔtreY ΔtreS (pEKEx3-ggpPS) during cultivation with 1% (w/v) sucrose as sole substrate in CgC minimal medium or minimal medium and 1.0 g L⁻¹ urea as sole nitrogen source

Medium	αGG production (mM)	Biomass (g cdm L⁻¹)	Substrate consumption (mM)	Yield P/S (mmol mol l⁻¹)
CgC-minimal medium	2.83 ± 0.37	4.33 ± 0.41	63.67 ± 2.86	44.40 ± 5.76
Minimal medium with 1.0 g L⁻¹ urea	3.91 ± 0.48	1.85 ± 0.06	31.48 ± 1.36	124.23 ± 15.40

Cultures were inoculated at an OD₆₀₀ of 1, α-GG synthesis was induced by addition of NaCl (final concentration 750 mM) after 4 h of cultivation. The fermentations were performed in triplicate, samples of each fermentation were analyzed in triplicate, means are from the three independent experiments; the indicated experimental error means standard deviation

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