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Table 4 Production of α-GG with different C. glutamicum strains carrying the plasmid pEKEx3-ggpPS during cultivation minimal medium with 1% (w/v) sucrose as sole substrate and 1.0 g L−1 urea as sole nitrogen source

From: Production of the compatible solute α-d-glucosylglycerol by metabolically engineered Corynebacterium glutamicum

Strain

αGG production (mM)

Biomass (g cdm L−1)

Substrate consumption (mM)

Yield P/S (mmol mol−1)

C. glutamicum (pEKEx3-ggpSP)

2.69 ± 0.46

2.34 ± 0.06

38.04 ± 0.98

70.9 ± 14.0

C. glutamicum IMglgA (pEKEx3-ggpSP)

5.24 ± 0.57

1.80 ± 0.01

35.70 ± 5.90

146.6 ± 16.1

C. glutamicum ΔotsA (pEKEx3-ggpSP)

5.54 ± 0.60

2.39 ± 0.03

35.24 ± 3.13

157.4 ± 17.0

C. glutamicum IMglgA ΔotsA (pEKEx3-ggpSP)

6.40 ± 0.88

2.20 ± 0.01

34.41 ± 4.36

186.1 ± 25.5

  1. Strains were inoculated at an OD600 of 1, α-GG synthesis was induced by addition of NaCl (final concentration 750 mM) after 4 h of cultivation. The fermentations were performed in triplicate, samples of each fermentation were analyzed in triplicate, means are from the three independent experiments; the indicated experimental error means standard deviation
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Microbial Cell Factories

ISSN: 1475-2859

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