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Table 3 Production of α-GG with different C. glutamicum strains carrying the plasmid pEKEx3-ggpPS during cultivation in CgC minimal medium with 1% (w/v) sucrose as sole substrate

From: Production of the compatible solute α-d-glucosylglycerol by metabolically engineered Corynebacterium glutamicum

Strain

αGG production (mM)

Biomass (g cdm L−1)

Substrate consumption (mM)

Yield P/S (mmol mol l−1)

C. glutamicum (pEKEx3-ggpSP)

0.57 ± 0.06

7.60 ± 0.03

60.82 ± 1.59

9.4 ± 1.1

C. glutamicum IMglgA (pEKEx3-ggpSP)

3.23 ± 0.15

5.31 ± 0.31

62.23 ± 2.63

52.0 ± 2.4

C. glutamicum ΔotsA (pEKEx3-ggpSP)

2.19 ± 0.20

4.91 ± 0.19

64.78 ± 2.74

33.8 ± 3.1

C. glutamicum IMglgA ΔotsA (pEKEx3-ggpSP)

4.22 ± 0.22

4.31 ± 0.23

61.19 ± 3.98

69.0 ± 3.6

  1. Strains were inoculated at an OD600 of 1, α-GG synthesis was induced by addition of NaCl (final concentration 750 mM) after 4 h of cultivation. The fermentations were performed in triplicate, samples of each fermentation were analyzed in triplicate, means are from the three independent experiments; the indicated experimental error means standard deviation
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Microbial Cell Factories

ISSN: 1475-2859

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