Table 2 Oligonucleotides used in this study
Oligonucleotide | Sequence | Purpose |
|---|---|---|
ggpS_5′_cgl | 5′-GCCTGCAGAGGAGAGGGCAAAAAATGAATTCATCCC-3′ | pEKEx2-GgpPS |
ggpS_3′_cgl | 5′-GCGGATCCTCACTTCACAGGTCAAGCTTAT-3′ | pEKEx2-GgpPS |
ggpP_5′_cgl | 5′-GCGGTACCAGGAGGAGGCTAATAATGGTATTACACCAACAAC-3′ | pEKEx2-GgpP |
ggpP_3′_cgl | 5′-GCGAATTCTTGCTAGTGGTGGTGGTGGTGGTGCTGGGAAAAA TGGACTCTTCGGCGCTGGGCCGCCTGCT-3′ | pEKEx2-GgpP |
otsA-for | 5′-GCTCTAGAGCATGGATGATTCCAATAGCTT-3′ | pASK-IBA3-otsA |
otsA-rev | 5′-GCCCTCGAGCGGTGAGTTTTCTCCCGACTGTG-3′ | pASK-IBA3-otsA |
glgA-IM-for | 5′-TGATGCGGCCGACAAATG-3′ | pDrive-IMglgA |
glgA-IM-rev | 5′-TGCGTGAGATCGCTGAAG-3′ | pDrive-IMglgA, analysis of glgA inactivation |
M13-FP | 5′-TGTAAAACGACGGCCAGT-3′ | analysis of glgA inactivation |