Fig. 4

Effect of nitrogen limitation on α-GG production with C. glutamicum (pEKEx3-ggpSP). Growth a, substrate consumption b and αGG accumulation c were determined for C. glutamicum (pEKEx3-ggpSP) in the course of cultivation in CgC-minimal medium (open squares), or CgC-minimal medium without ammonia and 0 g L⁻¹ urea (white circles), 0.1 g L⁻¹ urea (grey squares), 0.5 g L⁻¹ urea (solid diamonds), 1.0 g L⁻¹ urea (grey circles), or 5.0 g L⁻¹ urea (solid triangles). Data from one representative experiment of a series of three are shown