Development of a production chain from vegetable biowaste to platform chemicals

Table 1 Bacterial strains and plasmids used in this study

	Genotype	Source
Strains for BES
Geobacter sulfurreducens barcode strain	Synthetic sequence; 453226::kan barcode	[25]
Shewanella oneidensis barcode strain	Synthetic sequence; 71982::barcode	[25]
Escherichia coli		unpublished
Enterococcus faecium		unpublished
Shewanella putrefaciens		unpublished
Strains for production
E. coli K12 Δrnr (JW5741-1)	F⁻, Δ(araD-araB)567, Δ lacZ4787(::rrnB-3), λ⁻, rph-1, Δ(rhaD-rhaB)568, Δrnr-729::kan, hsdR514	[29]
Corynebacterium glutamicum (ATCC^® 13032™)
Plasmids
pMAL_alsSD	Amp^R, P_lac, alsSD	[30]

The barcode strains contain a short synthetic DNA sequence integrated in their genome that allows for the specific quantification of these organisms in mixed species communities [25]. The unpublished E. coli, E. faecium and S. putrefaciens strains were isolated previously from waste water as ferric iron reducing organisms (unpublished results)

ISSN: 1475-2859