Fig. 6

CPC production of Acppm1DM-mecBOE in the MDFA medium supplemented with varying concentrations of methionine. a The streamlined diagram of methionine cycle and cephalosporin biosynthesis. Hcy homocysteine, Cys cysteine, SAH S-adenosylhomocysteine, SAM S-adenosylmethionine, Met methionine, CPC cephalosporin C. The conversion of cystathionine to cysteine is catalyzed by cystathionine-γ-lyase (MecB). b The CPC production of Acppm1DM-mecBOE in the MDFA medium supplemented with 0, 0.32 and 3.2 g/L of methionine, respectively. CPC production was determined by bioassays against B. subtilis 1.1630 as described in “Methods”. Acppm1DM-mecBOE, overexpressing mecB in Acppm1DM. Error bars represent standard deviations from three independent experiments