Fig. 5

CPC production and intracellular SAM concentration in Acppm1DM. a CPC production of Acppm1DM in the MDFA medium supplemented with varying concentrations of methionine. One half (1.6 g/L), one-fifth (0.64 g/L), one-tenth (0.32 g/L), one-twentieth (0.16 g/L) and the normal concentration of methionine (3.2 g/L) were used in the MDFA medium for fermentation. b CPC production of WT, Acppm1DM, Acppm1CM and Acppm1OE in the MDFA medium supplemented with 0.32 g/L methionine. c Intracellular SAM concentration of WT and Acppm1DM. The concentrations of methionine supplemented in the MDFA medium were 3.2 g/L and 0.32 g/L, respectively. d CPC production of Acppm1DM with addition of exogenous SAM during fermentation. 4 μM and 1 mM of SAM were added after 3 or 4 days fermentation. Acppm1DM3d, SAM was added after 3 days fermentation; Acppm1DM4d, SAM was added after 4 days fermentation. WT, the wild-type strain; Acppm1DM, the Acppm1 disruption mutant; Acppm1CM, the complemented strain of Acppm1DM with a copy of Acppm1; Acppm1OE, the Acppm1 overexpressed strain. Met, methionine. CPC production was determined by bioassays against B. subtilis 1.1630 as described in the “Methods”. Error bars represent standard deviations from three independent experiments