Fig. 4

Transcription of 23 putative SAM dependent methyltransferase encoding genes (AcMTA-W) in A. chrysogenum. a Transcriptional analysis of AcMTA-W by semi-quantitation RT-PCR. Total RNA extraction and cDNA synthesis were performed as described in “Methods”. WT, the amplified products using cDNA isolated from WT which was grown in the MDFA medium at 28 °C for 5 days; WT + SAM, the amplified products using cDNA isolated from WT which was grown at 28 °C for 5 days and 500 μM of SAM was added in the MDFA medium at 4th day; +, the genomic DNA from WT as the positive control; −, the negative control. Transcription of the actin gene was used as control. b Transcriptional analysis of AcMTA-W by real-time RT-PCR. WT was grown at 28 °C for 5 days in the MDFA medium with or without addition of 500 μM of SAM after 4 days fermentation. Total RNA extraction and cDNA synthesis were performed as described in “Methods”. The relative transcriptional level of AcMTA-W was detected by real-time RT-PCR. The relative abundance of mRNAs was standardized against the transcriptional level of actin gene. A-W, the transcriptions of putative SAM dependent methyltransferase encoding genes AcMTA-W; actin, the transcription of actin gene. Error bars represent standard deviations from three independent experiments