Fig. 3

CPC production and intracellular concentration of SAM in WT and AcsamsOE. a CPC production in WT and AcsamsOE. CPC production of WT and AcsamsOE was determined by bioassays against B. subtilis 1.1630 as described in “Methods”. 3.2 g/L methionine was added in the MDFA medium when needed. b Intracellular concentrations of SAM in WT and AcsamsOE. Intracellular concentrations of SAM in WT and AcsamsOE were determined with the Enzyme-linked Immunosorbent Assay (ELISA) kit as described in “Methods”. The final concentration of methionine in the MDFA medium was 3.2 g/L. The concentration of total protein was 30 μg/μL. c CPC production of WT in the MDFA medium supplemented with 0, 0.4, 4.0 and 500 μM of SAM, respectively. d CPC production of WT in the MDFA medium supplemented with 4 μM and 1 mM of SAM after 3 or 4 days fermentation. The CPC production was determined by bioassays against B. subtilis 1.1630 as described in “Methods”. WT, the A. chrysogenum wild-type strain; AcsamsOE, the AcsamS overexpressed strain; Met, methionine; SAM S-adenosylmethionine. Error bars represent standard deviations from three independent experiments