Skip to main content
Fig. 2 | Microbial Cell Factories

Fig. 2

From: Metabolic engineering of Acremonium chrysogenum for improving cephalosporin C production independent of methionine stimulation

Fig. 2

CPC production and intracellular concentration of SAM in A. chrysogenum during fermentation. a CPC production of A. chrysogenum with or without addition of 3.2 g/L methionine. The CPC production of WT grown in MDFA with or without addition of methionine was determined by bioassays against B. subtilis 1.1630 as described in “Methods”. WT, the A. chrysogenum wild-type strain; Met, methionine. b Intracellular concentrations of SAM in A. chrysogenum during fermentation with or without addition of 3.2 g/L methionine. The concentration of SAM was determined with the Enzyme-linked Immunosorbent Assay (ELISA) kit as described in “Methods”. The concentration of total protein was 30 μg/μL. No SAM could be detected in both cultures after 5 days fermentation with or without addition of methionine. Error bars represent standard deviations from three independent experiments

Back to article page