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Table 1 Gradient elution profile for separation of theobromine and its metabolites

From: Bio-detheobromination of cocoa pod husks: reduction of ochratoxin A content without change in nutrient profile

Time (min)

Eluent mix (%)

Flow rate (ml/min)

0.1% ammonium formate

Acetonitrile

0

98

2

1.5

6

95

5

1.5

9

98

2

1.5

10

98

2

1.5

11

92

8

1.5

14

88

12

1.5

17

89

11

1.5

18

98

2

1.5

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Microbial Cell Factories

ISSN: 1475-2859

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