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Table 1 Strains and plasmids used in this study

From: Engineering Saccharomyces cerevisiae for the production of the valuable monoterpene ester geranyl acetate

Name

Description

Source

pUC57-GES

Cloning vector with a synthetic version of the GES gene from O. basilicum

GENEWIZ

pUC57-SAAT

Cloning vector with a synthetic version of the SAAT gene from cultivated strawberry (Fragaria × ananassa)

GENEWIZ

pEASY-Blunt

Cloning vector for blunt ligation

This study

pM3-GES

pEASY-Blunt vector with pTEF2-GES-tCYC1cassette

This study

pM2-SAAT

pEASY-Blunt vector with pPGK1-SAAT-tADH1 cassette

This study

pM4-ERG20(F96W–N127W)

pEASY-Blunt vector with pTDH3-ERG20(F96W–N127W)-tTPI1 cassette

This study

pM2-MAF1

pEASY-Blunt vector with pPGK1-MAF1-tADH1 cassette

This study

pM3-IDI1

pEASY-Blunt vector with pTEF2-IDI1-tCYC1cassette

This study

pM4-tHMG1

pEASY-Blunt vector with pTDH3-tHMG1-tTPI1 cassette

This study

pRS313-TRP

pRS313 vector: HIS selection marker was replased withTRP

This study

pRS313-GPPSAt

pRS313-TRP vector with pPGK-GPPSAt-tCYC1cassette

This study

pRS313-GPPSMp

pRS313-TRP vector with pPGK-GPPSMp-tCYC1cassette

This study