Table 1 List of E. coli strains and plasmids used in this work
From: fabH deletion increases DHA production in Escherichia coli expressing Pfa genes
Relevant characteristics | References | |
|---|---|---|
Strains | ||
BW25113 | F-, DE(araD-araB)567, lacZ4787(del)::rrnB-3, LAM-, rph-1, DE(rhaD-rhaB)568, hsdR514 | [16] |
BW27783 | BW25113 DE(araFGH) Φ(∆araEp PCPA–araE) | [19] |
Plasmids | ||
pDHA4 | pSTV29 (Takara Corp.) carrying pfaABCDE from M. marina MP-1 (p15A OriV, ~ 10 copies, chloramphenicol resistance cassette) pfaABCD expression is controlled by native M. marina promoters, pfaE is controlled by T7 promoter | [7] |