Fig. 2

Effect of fabH deletion and cerulenin on heterologous DHA production in E. coli strains cultured at 15 °C for 72 h. a Effect of fabH deletion on fatty acid composition of BW-pDHA4 and ∆FabH-pDHA4 in absence of cerulenin (untreated strains). b Effect of cerulenin on BW-pDHA4 and ∆FabH-pDHA4 in presence of 1 μg/ml cerulenin (treated strains). c Additive effect of fabH deletion and cerulenin treatment on fatty acid composition. Fatty acids from ∆fabH-pDHA4 treated with 1 μg/ml cerulenin are compared to fatty acids of untreated BW-pDHA4. Mean and standard deviation of each FA in relation to total FA content is shown. Statistical analysis was made for at least three replicates using One-Way ANOVA. Asterisks indicate the degree of significance (***p < 0.0001; **p < 0.001; *p < 0.05; ns not significant p > 0.05). Miristic acid—C14:0, palmitic acid—C16:0, palmitoleic acid—C16:1, C17 cyclopropane derivative—C17cyc, cis-vaccenic acid—C18:1n7, DHA—C22:6n3. d Growth curve of BW-pDHA4 and ∆FabH-pDHA4 with 0 and 1 μg/ml of cerulenin. Each curve was measured for 52 h at 15 °C using 12 independent cultures