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Table 2 Oligonucleotides used in this study

From: Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of d-glucose and l-arabinose

Name

Target gene

Sequence (5′-3′)

Cohesive endsa

Primer 1

gltA

CTCTGGATCCGACTACTTCCGTAATCCGGA

BamHI

Primer 2

gltA

CTCTCTGCAGCCGGTAGCTCAATCTGTGGC

PstI

Primer 3

pyk

CTCTGGATCCATGGTAGTACCTGTGGCTTG

BamHI

Primer 4

pyk

CTCTCTGCAGATGCTCTGCTCAAGAAGTGC

PstI

Primer 5

pfk

CTCTGGATCCATAAGATGGTCAGAGACAGT

BamHI

Primer 6

pfk

CTCTGAGCTCAGTCAAGCCTAGGTCACAGT

SacI

Primer 7

pCH

GGATCCGATATCCTGCAGGAG

 

Primer 8

pCH

CTCGACCAACAGTTGCGCAGC

 

Primer 9

araR

CTCTCTGCAGGCGCTCAATGCTTGACAGCG

PstI

Primer 10

araR

CTCTAAGCTTCCGACGGCATCTACACCGAT

HindIII

Primer 11

araR

CTCTGAATTCTCGTGGAGGTTTCGCAGGAA

EcoRI

Primer 12

araR

CTCTCTGCAGGCTGCCAATGACCAGATGGC

PstI

  1. a The restriction site overhangs used in the cloning procedure are underlined