Fig. 6

Time course metabolomics of aerobically growing wild-type C. glutamicum ATCC 31831 (black diamonds) and recombinant strains overexpressing pyruvate kinase in the absence or presence of araR deletion (31831/pCHpyk, blue triangles; ΔaraR/pCHpyk, red circles). Relative abundances of metabolic intermediates in cells during aerobic growth in BT medium containing a mixture of d-glucose and l-arabinose are shown. The X-axis indicates cultivation time (h). For the profiling experiment, three strains were grown aerobically to late log phase in A medium containing 20 g/L of l-arabinose and 20 g/L of d-glucose, and the culture was then inoculated to an initial OD₆₀₀ of 0.2 into BT medium containing a mixture of d-glucose and l-arabinose as the carbon sources (15 g/L each). The cells were harvested at 11, 17, and 23 h of cultivation and subsequently subjected to metabolome analysis. Data are presented as average ± standard deviation calculated from the results of triplicate individual experiments. The concentration of erythrose-4-phosphate was below the detection limit