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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of d-glucose and l-arabinose

Fig. 2

Consumption of l-arabinose and/or d-glucose by wild-type strain ATCC 31831 in mineral salt medium containing l-arabinose and d-glucose individually (a) or collectively (b). The wild-type strain was grown aerobically to late log phase in A medium (containing 20 g/L of l-arabinose and 20 g/L of d-glucose) and then inoculated to an initial OD600 of 0.2 into mineral salt BT medium. Using only a single carbon source (a), either l-arabinose or d-glucose was added to BT medium (15 g/L; solid symbols). Using two carbon sources (b), a mixture of l-arabinose and d-glucose was added to BT medium to final concentrations of either 3.6 g/L (open symbols) or 15 g/L (solid symbols). Cells were growth on either l-arabinose (reverse triangles) or d-glucose (diamonds) as single carbon sources, and also as a sugar mixture (squares). The concentrations of l-arabinose (empty triangles) and d-glucose (empty circles) are indicated. Data are plotted as the average ± standard deviation calculated from the results of triplicate individual experiments

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