Fig. 1

Construction of the minimal transcriptional activators. Plasmids for two sets of novel minimal transcriptional activators fusing the DNA-binding domains of ACEII or CREI with the VP16 activation domain and a short flexible linker (GGGGSGGGGS). Transformants T_MTA58/66/81 and T_{MTA96/109/139} were obtained after xylose-induced marker rescue. The natural factors were replaced by the minimal transcriptional activators in transformants genomes