Table 2 List of strains constructed in this work

ANG1

Control strain with empty plasmid (URA3)

ANG2

Contains expression cassettes of ssfE/ssfN/ssfK/ssfJ (URA3)

ANG3

Contains expression cassettes of ssfE/ssfN/ssfK/ssfJ (URA3) and an empty plasmid (LEU2)

ANG4

Contains expression cassettes of ssfE/ssfN/ssfK/ssfJ (URA3) and prpE (LEU2)

ANG5

Contains expression cassettes of pccB/accA1/ssfN/ssfK/ssfJ (URA3) and prpE (LEU2)

ANG6

Contains expression cassettes of ssfE (URA3) and prpE (LEU2)

ANG7

Contains expression cassettes of ssfE/ssfN (URA3) and prpE (LEU2)

ANG8

Contains expression cassettes of ssfE/ssfN/ssfK and prpE (LEU2)

ANG9

Contains expression cassettes of prpE (LEU2) and an empty plasmid (URA3)

ANG10

Contains expression cassettes of matB (LEU2) and an empty plasmid (URA3)

ANG11

Contains expression cassettes of ssfN/ssfK/ssfJ (URA3) and an empty plasmid (LEU2)

ANG12

Contains expression cassettes of ssfN/ssfK/ssfJ (URA3) and matB (LEU2)

ANG13

Contains expression cassettes of ssfE/thgK/thgH/thgI (URA3) and prpE (LEU2)

ANG14

Contains expression cassette of CoA ligase from Humulus lupulus (LEU2)

ANG15

Contains expression cassette of CoA ligase from Solanum tuberosum (LEU2)

ANG16

Contains expression cassette of CoA ligase-1 from Euphorbia peplus (LEU2)

ANG17

Contains expression cassette of CoA ligase-2 from Euphorbia peplus (LEU2)

ANG18

Contains expression cassette of CoA ligase-3 from Euphorbia peplus (LEU2)

ANG19

Control strain with empty plasmid (LEU2)

ANG20

Contains expression cassettes of ssfE/ssfN/ssfK/ssfJ (URA3), prpE (LEU2) and an empty plasmid (HIS3)

ANG21

Contains expression cassettes of ssfE/ssfN/ssfK/ssfJ (URA3), prpE (LEU2) and an additional copy of ssfE (HIS3)