Fig. 6
From: Biosynthesis of angelyl-CoA in Saccharomyces cerevisiae
AN-CoA production in strains expressing plant acyl-CoA ligases and fed with angelic acid. a Angelate enters the cell and is converted to angelyl-CoA by carboxyl CoA ligases. b Relative amount of AN-CoA accumulating in yeast strains expressing CoA-ligases HlCCL4 (ANG14), StCCL (ANG15), EpCCL1 (ANG16), EpCCL2 (ANG17), and EpCCL3 (ANG18). Strains were cultured in selective SC medium supplemented with 0.1 g/L angelic acid. c Time course of production of AN-CoA (open squares) and Ac-CoA (open triangles) by the strain expressing CoA-ligase StCCL (ANG15). The corresponding OD600 values are represented by filled circles. Engineered strains were incubated in selective SC medium supplemented with 0.1 g/L angelic acid (all data: mean ± SD, n = 3)