Fig. 4

Intracellular accumulation of Pr-CoA, MM-CoA, HMB-CoA, AN-CoA in strains expressing parts of the AN-CoA pathway. MAA-CoA could not be found in any of the strains. Pr-CoA (purple bars), MM-CoA (yellow bars), HMB-CoA (red bars) and AN-CoA (green bars) in strains expressing prpE (ANG9), prpE + ssfE (ANG6), prpE + ssfE/ssfN (ANG7), prpE + ssfE/ssfN/ssfK (ANG8) and prpE + ssfE/ssfN/ssfK/ssfJ (ANG4). Engineered strains were incubated for 12 h in selective SC medium buffered to pH 4.5 and supplemented with 0.5 g/L propionic acid. Represented are the averages and standard deviations of three independent cultures