Engineering xylose metabolism for production of polyhydroxybutyrate in the non-model bacterium Burkholderia sacchari

Table 3 Summary of the results of P(3HB) production and xylose growth rate in B. sacchari overexpressing xylR or rfp

Plasmid	CDW (g/L)	%P(3HB)	P(3HB) (g/L)	µmax (h⁻¹)	Y_xyl/P(3HB) (g/g)
pF8k-xylR	7.15 ± 0.58	65 ± 1.98	4.65	0.20 ± 0.003	0.348 ± 0.007
pF8k-RFP	5.12 ± 0.72	51.95 ± 3.01	2.66	0.156 ± 0.009	0.237 ± 0.005
pF5k-xylR	8.02 ± 0.44	71.07 ± 2.46	5.7	0.252 ± 0.01	0.396 ± 0.009
pF5k-RFP	5.34 ± 0.66	54 ± 2.88	2.89	0.162 ± 0.016	0.225 ± 0.006

Shake flask experiments with mineral medium supplemented with xylose (15 g/L). Cell dry weight (CDW), polymer content of CDW [P(3HB)], P(3HB) yield from xylose (Y_xyl/P(3HB)), and P(3HB) volumetric productivity [P(3HB)] are presented. CDW was determined gravimetrically, carbohydrates by HPLC [49], and percentage of P(3HB) by gas chromatography of propyl esters [33]. Presented data correspond to averages from triplicate experiments. Standard deviation about those averages are also presented

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