Table 1 Primers designed in the present study to amplify the genes of interest for cloning in the compatible BglBrick plasmid
Name | Sequence | Restriction site |
|---|---|---|
xylA_F | AAAAAAGAATTCTTTAAGAAGGAGATATACATATGGGAGCCGAGCGCAAA | EcoRI |
xylB_R | AAAAAAGGATCCTCAGGCGCCGGATGCGAATA | BamHI |
xylF_F | AAAAAACATATGAAGTTCGCCAAACGCCGTTCG | NdeI |
xylH_R | AAAAAACTCGAGTCAGCGGCGGTTCGACCCC | XhoI |
xylE_F | AAAAAAAGATCTTTTAAGAAGGAGATATACATATGACCGCGCTGGAAAAGAAAG | BglII |
xylE_R | AAAAAACTCGAGTCAGTGTGAAAACCCGGAGGCCG | XhoI |
xylR_F | AAAAAAAGATCTTTTAAGAAGGAGATATACATATGACCCGCGCCACCGCTTCCC | BgIII |
xylR_R | AAAAAACTCGAGTCAGGAGTTTGATGTGCATGCGGCGCGC | XhoI |
EcoRI-BglII_pF8K_F | AAAAAAGAATTCAAAAGATCTTTTAAGAAGGAGATATACATATGGCGAG | EcoRI, BglII |
Prom_XbaI_pF8K_R | AAAAAATCTAGAGGAGAAACAGTAGAGAGTTGCGATAAAA | XbaI |
XbaI_RBS_xylE_F | AAAAAATCTAGATTTAAGAAGGAGATATACATATGACCGCGCTGGAAAAGAAAG | XbaI |
xylESTOP_BglII_R | AAAAAAAGATCTTCAGTGTGAAAACCCGGAGGCCG | BglII |
XbaI_RBS_xylA_F | AAAAAATCTAGATTTAAGAAGGAGATATACATATGGGAGCCGAGCGCAAA | XbaI |
xylBSTOP_EcoRI_R | AAAAAAGAATTCTCAGGCGCCGGATGCGAATA | EcoRI |
XbaI_RBS_xylE_F | AAAAAATCTAGATTTAAGAAGGAGATATACATATGACCGCGCTGGAAAAGAAAG | XbaI |
xylESTOP_BglII_R | AAAAAAAGATCTTCAGTGTGAAAACCCGGAGGCCG | BglII |
XbaI_RBS_xylA_F | AAAAAATCTAGATTTAAGAAGGAGATATACATATGGGAGCCGAGCGCAAA | XbaI |
xylBSTOP_EcoRI_R | AAAAAAGAATTCTCAGGCGCCGGATGCGAATA | EcoRI |