Table 3 Genes and induction conditions leading to the highest obtained hydroxybenzoate product titers in C. glutamicum DelAro5
From: Corynebacterium glutamicum as platform for the production of hydroxybenzoic acids
Product | DAHP synthase | Codon-optimization | Hydroxybenzoate-forming enzyme | Codon-optimization | IPTG concentration (µM) | Product titer (g/L) |
|---|---|---|---|---|---|---|
protocatechuate | aroF* | Yes | qsuB | Noa | 20 | 1.47 |
2-hydroxybenzoate | aroF* | Yes | irp9 | Yes | 20 | 0.01 |
3-hydroxybenzoate | aroF* | Yes | hyg5 | Yes | 40 | 0.26 |
4-hydroxybenzoate | aroH | No | ubiC | No | 1000 | 2.32 |